منابع مشابه
Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins.
IE1, a principal transcriptional activator of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), is an essential factor for viral DNA replication. During viral infection, IE1 accumulates in discrete subnuclear structures where viral DNA replication occurs. To analyse the dynamic properties of IE1, we monitored green fluorescent protein-tagged IE1 (IE1-GFP) in BmNPV-infected B. mori cells...
متن کاملExpression and structural characterization of a baculovirus ecdysteroid UDP-glucosyltransferase.
The baculovirus enzyme ecdysteroid UDP-glucosyltransferase (EGT) disrupts the hormonal balance of the insect host by catalysing the conjugation of ecdysteroids, the moulting hormones, with the sugar moiety from UDP-glucose or UDP-galactose. In this study, EGT has been overproduced using a recombinant Autographa californica nucleopolyhedrovirus and an antiserum has been raised against the purifi...
متن کاملProtection of proteolysis-prone recombinant proteins in baculovirus expression systems.
Since its introduction in 1983 (11), the baculovirus system has become widely used in studies on eukaryotic proteins, especially those synthesized in the rough endoplasmic reticulum. In a few cases, heterogeneity has been observed in proteins produced in Spodoptera cells, pointing to limited proteolysis such as amyloid precursor protein (9), interferon α2 (12) and muscarinic M2 receptor (3). In...
متن کاملDiagnostic potential of baculovirus-expressed rubella virus envelope proteins.
The envelope glycoproteins E1 and E2 of rubella virus were abundantly expressed in Spodoptera frugiperda Sf9 insect cells by using a baculovirus expression vector. The recombinant protein products were purified by immunoaffinity chromatography and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The purified recombinant an...
متن کاملExpression of polyisoprenylated Ras proteins in the insect/baculovirus system.
Kus proteins and many related proteins possess the C-terminal motif C‘ys-AAX. where A is an aliphatic ;imino acid and X is itny iiniino acid. ‘I’his sequence directs ii prenyltransferiise. fh-nesyltransferase in the c;ise of ltas and geranylger;inyItransferase in most other ltas-related proteins. t o prenylate the cysteine residue 11-01. The nitture of the amino acid residue X appears t o deter...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1992
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-73-4-749